Animal Nutrition

Mycotoxin levels in soybean meals

To read more content about aviNews International December 2023

Anneliese Mueller

Bettina Behler-Wöchtl

Edgar O. Oviedo-Rondón

Mycotoxin evaluation is frequent in feed mills for grain sources like corn, wheat, and barley or products like peanuts and cottonseed.

However, soybean meal, the most common protein source in feed for all animal species, needs more attention in this critical quality aspect.

  • Several studies have indicated that soybean meal appears to be less susceptible to mycotoxin contamination than most grains.

However, significant differences can be observed in contamination with mycotoxins among sources of soybean meal.

Previous reports have indicated that the main mycotoxins eventually observed in soybean meals can be:

However, several publications suggest some variability per region. That effect should be clarified to determine if contamination relates with:

  • The location where samples were collected or
  • The origin of production and manage risks according to these factors.

Global evaluations of samples are necessary since soybean meals have considerable trade worldwide. Relevant differences in nutrient and processing quality have been reported among countries of origin.

The data on mycotoxin levels in soybean meal presented in this article are part of the dsm-firmenich World Mycotoxin Survey.

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Most results included in the survey are obtained using methods like ELISA, HPLC, or state-of the art LC-MS/MS-based multimycotoxin methods.

Samples that tested positive for one of the main mycotoxins are also presented as the percentage of contaminated

The average and median of positives are calculated in ppb of all positively tested samples. The maximum levels are also described.

Such high levels are seldom observed, but still, they occur and underline the importance of testing for mycotoxin occurrence.


China, the United States, Brazil, Argentina, and India are leading soybean meal producers. The 27 European Union
countries may produce more soybean meal together than India, but not as individual countries.

Considering the production volume and the data in the dsm-firmenich World Mycotoxin Survey that can track the origin of the soybean meal, we looked at the results of soybean meal samples from China, USA, Brazil, Argentina, and India analyzed from June 2022 to June 2023.


The results of this study are summarized in the following Table and the prevalence of each mycotoxin per country in the Figures.

The contamination levels of all soybean samples worldwide are always lower than reported in grains, confirming what scientific papers have indicated.

The most significant number of samples evaluated were from Argentina and Brazil, and the lowest was from India.

In China (Figure 1), Argentina (Figure 4), and India (Figure 5), 86% or 82% of the samples were positive for ZEN; however, in India, 97% of the samples analyzed were positive for OTA and 92% positive for T2 (Figure 5).

United States soybean meal (Figure 2) had the lowest percentage of samples positive for ZEN (28%) among all five countries studied and the lowest average and median concentration (17 and 15 ppb) or maximum level (38 ppb) of ZEN.

In all other countries, the ZEN levels observed were almost three times (2.3 to 3.6) higher. The highest levels of ZEN (Table 1) were observed in samples from Argentina and Brazil (1000 and 809 ppb).

Aflatoxins were the second most common mycotoxin observed in Argentinian soybean meals, with 63% of the 1,459 samples evaluated being positive (919 samples).

However, contamination levels were around 2-3 ppb in Argentinian soybeans, and similar levels were observed in the Chinese and Indian soybean meals, with 29 and 26% of the samples positive for Afla.

A few samples from Brazil (13 out of 673) and the USA (2 out of 43) had Afla (Figures 3 and 2).

However, the median concentration of Afla in Brazilian samples was 4 ppb. In comparison, the two USA samples reached a median of 45 ppb, which could be considered outliers and hard to observe again.

No samples of Brazilian soybean meal were positive for OTA (Figure 3), indicating low or no contamination with Penicillium molds in these samples.

No T2 was observed in the Chinese samples (Figure 1), but 46% (26 out of 56) were positive for DON.

The United States soybean meals positive for mycotoxins generally had the lowest average and median concentrations of DON, T2, FUM, and OTA observed worldwide (Table 1).


Based on the median concentration of these mycotoxins and the number of positive samples within the survey, the United States soybean meal is currently the safest source to avoid multi-mycotoxin contamination.

However, monitoring mycotoxins in all soybean meals every year is always advisable because concentrations of mycotoxins may occur due to annual weather fluctuations.



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