Diagnostic tests
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Infectious bronchitis is a highly relevant disease in all poultry production countries. This disease is caused by the infectious bronchitis virus (IBV), a coronavirus that shows continuous mutations and recombinations.
Infectious bronchitis is mainly associated with respiratory problems, although kidney problems have also been described.
CASE
Three animals with severe respiratory symptoms were sent to the laboratory. On examination, the animals were 0.5 kg below standard bodyweight.
The necropsy reveals that the birds had:
The necropsy and anamnesis findings are nonspecific but could be compatible with an infectious bronchitis process. The first signs of the disease are typical upper respiratory tract disease (coughing, sneezing, head shaking…), these being more common in young birds. Infection can lead to growth delays, and some birds may die suddenly from bronchial occlusion.
In addition, it is usually accompanied by a marked drop in laying (10-15%), poor quality eggs (liquid albumen, fragile and depigmented eggshell …), ovary degeneration, and inflammation of the oviducts. Mortality rates are generally higher in the presence of secondary infections. A colibacillosis due to a secondary Escherichia coli infection can manifest itself in different ways in birds, with colisepticemia being the most characteristic sign.
Diagnostic tests
For the diagnosis, serological analysis of the animals (indirect test), microbiological and molecular analyses (PCR) were performed as a diagnostic test to detect the causal agent.
Serological studies are mainly carried out to identify whether animals have developed antibodies against a pathogen, both in the field and in the vaccine.
The microbiological analysis was performed from