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The use of vaccines in the incubator is increasingly frequent, with broiler chicken integrations that all their vaccination program is already done only in the incubator, even in the case of breeders and layers the incubator immunization covers more and more diseases. The application is made both at 18-19 days of incubation, by application in ovo , and at the day of life by nebulization or subcutaneous injection.
Most of the new vaccines have been designed for application in the incubator , which has advantages over farm application. The vaccination procedure is better controlled and more sophisticated and precise vaccination equipment is used both in the dosage and distribution of the vaccine, better hygienic conditions in the preparation and reconstitution of the vaccine and earlier immunization.
This forces us to have various controls from the incubator to ensure a correct vaccination:

Conditions of conservation
Preparation process control
Vaccine Compatibility
Control of vaccination sessions
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Conditions of conservation
Based on the storage conditions, we handle two different types of vaccines :
Vaccines frozen in nitrogen . It should be noted that two types of containers are used for the conservation of these vaccines:
With liquid nitrogen: we must check at least twice a week that the nitrogen level is adequate so that all the ampoules are submerged in the nitrogen and fill it if necessary. It is convenient to keep a record with the checks that are being made.
With dry nitrogen: we should not use these containers for more than 14 days, so it is necessary to write the date of receipt of these containers.

The vaccine vials are placed face down on the rods that are introduced into the baskets of the nitrogen container, this helps us to detect if at any time the vaccine has been partially or totally thawed. Whenever we find vaccine in the cap of the vial we must discard it.

Refrigerated vaccines In this group would be both lyophilized and coccidia vaccines. The recommendations of the laboratories is to keep these vaccines between 2 and 8º C, for which we can use a refrigerator. In this case it is interesting to have a thermometer that records the maximums and minimums inside the refrigerator to ensure that the storage conditions of the vaccine are ideal. Taking care that the vaccine does not contact the bottom wall of the refrigerator that can cause freezing.
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